目的 研究人参糖肽(GGP)对糖尿病气阴两虚模型大鼠降血糖作用及其机制。方法 造模组大鼠喂养高脂饲料4周后再腹腔注射链脲佐菌素(STZ),根据血糖值随机分为模型对照组、人参糖肽高剂量组(200 mgkg-1)、人参糖肽中剂量组(100 mgkg-1)及人参糖肽低剂量组(50 mgkg-1),将同批未经上述处理的大鼠作为正常对照组,每组10只大鼠。将上述4组糖尿病大鼠每天灌胃给予过量的青皮(15 g)、附子(10 g)建立2型糖尿病气阴两虚证模型,期间人参糖肽高、中、低剂量组每天皮下注射给药,正常对照组与模型对照组皮下注射同体积生理盐水,每天1次,连续4周。给药期间,糖尿病大鼠均继续喂以高脂饲料及灌服青皮、附子。每周测血糖1次,末次给药结束后,禁食12 h,乙醚麻醉,腹主动脉取血,检测气阴两虚糖尿病大鼠血清中血糖(Glu)、胆固醇(TC)、甘油三酯(TG)、糖化血红蛋白(GHb)、胰岛素(Ins)、C肽(C-P)、超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽(GSH)、肿瘤坏死因子(TNF-α)、游离脂肪酸(FFA)及一氧化氮(NO)水平。结果 与正常对照组大鼠比较,模型对照组大鼠血糖、甘油三酯、胆固醇、丙二醛、一氧化氮、肿瘤坏死因子、游离脂肪酸、糖化血红蛋白水平明显升高(P<0.05或P<0.001),超氧化物歧化酶、谷胱甘肽、胰岛素、C肽、肝糖原含量明显降低(P<0.05或P<0.001)。与模型对照组比较,人参糖肽高、中、低剂量组血糖水平均明显降低(P<0.05或P<0.01);人参糖肽高剂量组与人参糖肽中剂量组大鼠甘油三酯、胆固醇、丙二醛、一氧化氮、肿瘤坏死因子、游离脂肪酸、糖化血红蛋白水平明显降低(P<0.05或P<0.001),超氧化物歧化酶、谷胱甘肽、胰岛素、C肽、肝糖原含量明显升高(P<0.05或P<0.001);人参糖肽低剂量组大鼠肿瘤坏死因子及糖化血红蛋白水平明显降低(P<0.05),超氧化物歧化酶、谷胱甘肽、胰岛素及C肽含量明显升高(P<0.05),但对其他指标无显著性差异(P>0.05)。结论 人参糖肽对糖尿病气阴两虚大鼠具有显著的降血糖作用,可明显改善糖尿病气阴两虚大鼠糖脂代谢及抑制胰岛素抵抗。
Abstract
OBJECTIVE To study on hypoglycemic effect and mechanism of the ginseng glycopeptide (GGP) in diabetic rats with QI and Yin deficiency.METHODS The model of type 2 diabetic rats was induced by high-fat diet feeding for 4 weeks combined with intraperitoneal injection of streptozotocin(STZ).Then the rats without above-mentioned treatment were selected as the normal control group, the diabetic rats were randomly divided into four groups by blood glucose, the high (200 mgkg-1) dose of the GGP group, the medium (100 mgkg-1) doses of the GGP group, low (50 mgkg-1) doses of the GGP group. Excessive dose of Pericarpium Citri Reticulatate Viride (15 g) and Radix Aconity Praeparata(10 g) were administered into rats by po to establish models of Qi and Yin deficiency in type 2 diabetes mellitus. The rats in GGP groups were subcutaneously injected with GGP preparation for 4 weeks. The model control rats and normal control rats were treated by subcutaneous injection of saline for 4 weeks. The rats were fed with high fat diet and Pericarpium Citri Reticulatate Viride and Radix Aconity Praeparata for during treatment. The glucose(Glu), total cholesterol (TC), triglyceride (TG), glycated hemoglobin(GHb), serum insulin(Ins), C-peptide(C-P), superoxide dismutase(SOD), malondialdehyde(MDA), glutathione(GSH), tumor necrosis factor-α(TNF-α) free fatty acid (FFA), and nitric oxide (NO) were measured at the end of the experiment. RESULTS Compared with the normal group, the levels of Glu, TC, TG, FFA, TNF-α, NO, MDA and GHb were increased obviously (P<0.05 or P<0.01), the levels of Ins, C-P, SOD and GSH were significantly decreased (P<0.05 or P<0.01). Compared with the model group, the levels of Glu, TC, TG, FFA, TNF-α, NO, MDA and GHb were significantly decreased (P<0.05 or P<0.01), the levels of Ins, C-P, SOD and GSH were increased obviously in the high-dose group and medium-dose group (P<0.05 or P<0.01). The level of TNF-α were significantly decreased (P<0.05), the levels of SOD, GSH, Ins and C-P were increased significantly (P<0.05) in the low-dose group. The other indexes were no significant difference (P>0.05). CONCLUSION GGP can significantly decrease the level of blood glucose, significantly improve the glucose and lipid metabolism and inhibit insulin resistance in rats with QI and Yin deficiency.
关键词
人参糖肽 /
链脲佐菌素 /
降血糖 /
胰岛素抵抗 /
气阴两虚
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Key words
ginseng glycopeptide /
streptozotocin /
hypoglycemic /
insulin resistance /
QI and Yin Deficiency
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中图分类号：
R965
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参考文献
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